Free radical scavenging activity of the methanolic crude leaf extracts of cananga odorata and jasminum sambac / Section 1-A2; Hena Alcantara.

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Language: english Publication details: Fairview, Quezon City: Department of Biochemistry, FEU-NRMF, 2013.Description: 36 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

M BIO 2013 0004

Summary: Abstract: Free radicals are constantly generated and may cause extensive damage to tissues and biomolecules leading to various ill conditions, especially degenerative diseases, and extensive lysis of cells. Many synthetic drugs protect against oxidative damage but have adverse side effects. An alternative solution to the problem is consuming natural antioxidants from supplements and also traditional medicine. Examples of these are Jasminum sambac and Cananga odorata. These were known as folklore medicine for centuries and no local paper has established their biochemical properties. Antioxidant screening was done by preparing crude methanolic extracts of the J. sambac and C. odorata leaves and ascorbic acid, which were subjected to DPPH scavenging assay using a series of dilutions and run into a spectrophotometer at 505 nm. At the highest concentration of 250µg/ml., J. sambac and C. odorata methanolic crude leaf extracts each have a %Inhibition of 71.97% and 77.78% respectively, while the positive control (ascorbic acid) has a %Inhibition of 94.40%. Based on the Student T-test statistical analysis of the %Inhibition obtained, both samples showed that antioxidant property and capacity of the crude leaf extracts were statistically comparable (p >0.05) with ascorbic acid. The IC50 for both crude leaf extracts of J. sambac and C. odorata of 156.40 and 106.18 µg/mL respectively, were higher than that of ascorbic acid.

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Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	M BIO 2013 0004 (Browse shelf(Opens below))	Available		T000993

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Includes appendices and bibliographical references.

Abstract: Free radicals are constantly generated and may cause extensive damage to tissues and biomolecules leading to various ill conditions, especially degenerative diseases, and extensive lysis of cells. Many synthetic drugs protect against oxidative damage but have adverse side effects. An alternative solution to the problem is consuming natural antioxidants from supplements and also traditional medicine. Examples of these are Jasminum sambac and Cananga odorata. These were known as folklore medicine for centuries and no local paper has established their biochemical properties. Antioxidant screening was done by preparing crude methanolic extracts of the J. sambac and C. odorata leaves and ascorbic acid, which were subjected to DPPH scavenging assay using a series of dilutions and run into a spectrophotometer at 505 nm. At the highest concentration of 250µg/ml., J. sambac and C. odorata methanolic crude leaf extracts each have a %Inhibition of 71.97% and 77.78% respectively, while the positive control (ascorbic acid) has a %Inhibition of 94.40%. Based on the Student T-test statistical analysis of the %Inhibition obtained, both samples showed that antioxidant property and capacity of the crude leaf extracts were statistically comparable (p >0.05) with ascorbic acid. The IC50 for both crude leaf extracts of J. sambac and C. odorata of 156.40 and 106.18 µg/mL respectively, were higher than that of ascorbic acid.

Thesis - Department of Biochemistry & Nutrition

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