THE EFFECT OF METFORMIN, TROGLITAZONE AND COMBINATION OF BOTH DRUGS IN LOWERING THE BLOOD GLUCOSE LEVEL OF A THIAZIDE - INDUCED HYPERGLYCEMIC MALE ALBINO RATS

ABSTRACT: Diabetes Mellitus, the most common endocrine disease, is characterize by metabolic abnormalities and by long term complication involving the eyes, kidney, nerves, and blood vessels. It occurs when circulating insulin concentrations decline or when the target cells become resistant to the hormone. Most cases can be divided into two types: a juvenile-onset (IDDM) and mature-onset (NIDDM). This study however, focuses on the treatment of the second type of Diabetes Mellitus, which is the non-insulin dependent Diabetes Mellitus. Several classes of oral hypoglycemic agents has been identified but only one, the sulfonylures, is recurrently used. But a related agent, Metformin, has been intoduced, in combination with said sulfonylureas. Another class of drug, the thizolidinediones like Troglitazone, has been also approved by Bureau of Food and Drugs. Since NIDDM patient shows poor response to a single drug therapy, the study aims to compare the effect of Metformin, Troglitazone, and Metformin + Troglitazone in combination in lowering blood glucose levels. The study utilizes male albino rats in which thiazide was administered to make them hyperglycemics. The said rats were grouped randomly into four groups. Each designated as control (A), Metformin (B), Troglitazine (C), and Metformin + Troglitazone (D). Glucose level were measured using an accutes III glucometer utilizing a haemo glucose test strip. The results obtained were interpreted using ANOVA which give us a conclusion that Metformin, Troglitazone, and the combination of Metformin and Troglitazone are effective in lowering blood glucose level. But there was no significant difference among the three groups in lowering the said blood glucose level.

Department of Pharmacology