Image from Google Jackets

A COMPARATIVE STUDY ON THE HYPOGLYCEMIC EFFECT OF IPOMOEA AQUATICA (KANGKONG) LEAVES AND STEM CRUDE EXTRACT ON HYPERGLYCEMIA-INDUCED MALE SPRAGUE DAWLEY RATS

By: Contributor(s): Language: English Publication details: QUEZON CITY FEU-NRMF MARCH 2010Description: 55 PAGESLOC classification:
  • MPHAR20100001
Summary: ABSTARCT: The Sprague Dawley Rats undergone a 12 hour fasting with water after which they were induced with 50% dextrose to elicit a hyperglycemic effect Blood glucose levels were measured after the 12 hour fasting and 15 minutes after giving the 50% dextrose, which serve as the fasting blood sugar of the rats and the Baseline glucose levels, respectively. Administration of treatments to assigned groups of rats was done through the oral route using 3 ml disposable syringes. Group A were given the crude extract of the kangkong Ipomea aquatica leaf, Group B were given the crude extract of the kangkong Ipomea aquatica stem, Group C were given Metformin as the positive control and Group D were given 5 ml distilled water as the negative control. Glucose levels were monitored through the blood extraction from rat`s tail at time 1, 2 and 3 hours. Blood glucose levels were assayed with the use of a Glucometer, Acue-Check. The result shows that both extract (stem and leaves) had a decrease in blood glucose level and were consistent with the standard hypoglycemic agent Metformin (Positive group). The effective duration to induce hypoglycemic effect as established basing on Paired t-test result at 95% significance level only true for 2nd and 3rd hour for both plant parts. As one of the main objective of this study, the effective duration to induce hypoglycemic effect can be established basing on 95% significance level. All of the groups, Group A (leaves), Group B (stem), (+) Control and (-) Control showed a significant difference from the baseline with that of after treatment, 2nd hour up to the 3rd hour as shown by the table and in the results of paired T-test. The hypoglycemic effect was more pronounced on the 3rd hour on the four groups with p-values (>0.05): 17.998 (Group A), 16.400 (Group B), 7.646 (Group C), 11.275 (Group D). Independent T-test was also performed to determine the difference between the positive control with that of the Group A (leaves) and Group B separately, which apparently showed no significant difference with a p-value of 0.871(>0.05) and 0.008 (>0.05) respectively. It could be concluded that both leaf and stem extract of Ipomoea aquatic can lower blood glucose levels. It can also be concluded that the time at which the hypoglycemic action of the crude extract that look effect was on the 2nd hour but the effect is greatest on the 3rd hour after treatment.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Call number Status Date due Barcode
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MPHAR20100001c.2 (Browse shelf(Opens below)) Available T001748
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MPHAR20100001 (Browse shelf(Opens below)) Available T000706

ABSTARCT: The Sprague Dawley Rats undergone a 12 hour fasting with water after which they were induced with 50% dextrose to elicit a hyperglycemic effect Blood glucose levels were measured after the 12 hour fasting and 15 minutes after giving the 50% dextrose, which serve as the fasting blood sugar of the rats and the Baseline glucose levels, respectively. Administration of treatments to assigned groups of rats was done through the oral route using 3 ml disposable syringes. Group A were given the crude extract of the kangkong Ipomea aquatica leaf, Group B were given the crude extract of the kangkong Ipomea aquatica stem, Group C were given Metformin as the positive control and Group D were given 5 ml distilled water as the negative control. Glucose levels were monitored through the blood extraction from rat`s tail at time 1, 2 and 3 hours. Blood glucose levels were assayed with the use of a Glucometer, Acue-Check. The result shows that both extract (stem and leaves) had a decrease in blood glucose level and were consistent with the standard hypoglycemic agent Metformin (Positive group). The effective duration to induce hypoglycemic effect as established basing on Paired t-test result at 95% significance level only true for 2nd and 3rd hour for both plant parts. As one of the main objective of this study, the effective duration to induce hypoglycemic effect can be established basing on 95% significance level. All of the groups, Group A (leaves), Group B (stem), (+) Control and (-) Control showed a significant difference from the baseline with that of after treatment, 2nd hour up to the 3rd hour as shown by the table and in the results of paired T-test. The hypoglycemic effect was more pronounced on the 3rd hour on the four groups with p-values (>0.05): 17.998 (Group A), 16.400 (Group B), 7.646 (Group C), 11.275 (Group D). Independent T-test was also performed to determine the difference between the positive control with that of the Group A (leaves) and Group B separately, which apparently showed no significant difference with a p-value of 0.871(>0.05) and 0.008 (>0.05) respectively. It could be concluded that both leaf and stem extract of Ipomoea aquatic can lower blood glucose levels. It can also be concluded that the time at which the hypoglycemic action of the crude extract that look effect was on the 2nd hour but the effect is greatest on the 3rd hour after treatment.

Department of Pharmacology

There are no comments on this title.

to post a comment.