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DETERMINATION OF ACUTE ORAL TOXICITY OF EUPHORBIA HIRTA LINN (TAWA-TAWA) IN SPRAGUE DAWLEY RATS USING OECD 420 (FIXED DOSE PROCEDURE)

Contributor(s): Language: English Publication details: Quezon City FEU-NRMF March 2012Description: 53 pContent type:
  • Text
Media type:
  • Unmediated
Carrier type:
  • Volume
LOC classification:
  • MPHAR20120004
Summary: ABSTRACT: The objectives of the study are to determine the lethal dose (LD50) of Euphorbia hirta L. lyophilized extract on Sprague- Dawley rats using OECD 420 (Fixed Dose Procedure). Second, the researchers describe the acute toxidrome of Euphobia hirta L.(Tawa-Tawa) based on the changes in central nervous, respiratory, eyes, ears behavior and weight. Third, is to describe the gross and histolopathologic changes in Sprague Dawley rats in 3 days brought about by Euphorbia hirta L. The study used the Fixed Dose Procedure and Quasi-Experimental design. The leaves, stem, flower were subjected to decoction, a process where in the leaves where extracted by boiling. After decoction, it was lyophilized to obtain powdered form of the water-soluble components. The subjects were randomly chosen, one rat was used for the sighting study and five rats were used for the main study. The dose for the sighting study was 300mg/kg body weight and used 2000mg/kg body weight for the main study. The lyophilized Tawa-Tawa extract was tested on laboratory experimental rats and was given as a single dose via lavage. There were no incidence (0%) central nervous depression, on the other hand there were 100% increase in the respiratory rate and startle response, only 20% exhibited fine and course body tremors. Furthermore, only 20 % exhibited changes in the eyes, there were no significant findings in the ears, in autonomic changes, 20 % exhibited salivation and 100 % exhibited tail lashing, pilomotor erection and increase body temperature. For behavioural changes 100 % exhibited passivity and there were also an increased body weight with an average of 0.6 grams. The researchers conclude that the LD50 of Euphorbia hirta L. (tawa-Tawa) is greater than 2000 mg/kg/bw base on OECD 420. The extract has no significant toxic effect on the central nervous system, respiratory system, autonomic nervous system, general appearance of the eyes & ears, body weight and behaviour of Sprague Dawley rats. Also, the researchers conclude that there are no gross abnormality in the organs of Sprague Dawley rats including, heart, lungs, liver, kidneys, spleen, as well as in the small and large intestines. Lastly, the histopathologic results are not sufficient to conclude that there is no toxicity at a dose of 2000 mg/kg/bodyweight due to poor specimen quality.
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Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MPHAR20120004c.1 (Browse shelf(Opens below)) Available T000748
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MPHAR20120004c.2 (Browse shelf(Opens below)) Available T000749
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MPHAR20120002C.2 DETERMINATION OF THE EFFECTS OF COMBINED ENERGY DRINK AND ALCOHOL ON THE MOTOR FUNCTION OF MALE ICR WHITE MICE MPHAR20120003C.1 A COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITY OF GUAVA (PSIDIUM GUAJAVA, LINN.) LEAF EXTRACTS AND THE GENERIC DRUG OF AMOXICILLIN AGAINST STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI MPHAR20120003C.2 A COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITY OF GUAVA (PSIDIUM GUAJAVA, LINN.) LEAF EXTRACTS AND THE GENERIC DRUG OF AMOXICILLIN AGAINST STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI MPHAR20120004c.1 DETERMINATION OF ACUTE ORAL TOXICITY OF EUPHORBIA HIRTA LINN (TAWA-TAWA) IN SPRAGUE DAWLEY RATS USING OECD 420 (FIXED DOSE PROCEDURE) MPHAR20120004c.2 DETERMINATION OF ACUTE ORAL TOXICITY OF EUPHORBIA HIRTA LINN (TAWA-TAWA) IN SPRAGUE DAWLEY RATS USING OECD 420 (FIXED DOSE PROCEDURE) MPHAR20120005C.1 A COMPARATIVE STUDY ON THE EFFICACY OF TONGYITANG HYPOGLYCEMIC CAPSULE AND METFORMIN - GLIBENCLAMIDE IN REDUCING BLOOD GLUCOSE LEVELS OF GLUCOSE - INDUCED HYPERGLYCEMIA IN RATS MPHAR20120005C.2 A COMPARATIVE STUDY ON THE EFFICACY OF TONGYITANG HYPOGLYCEMIC CAPSULE AND METFORMIN - GLIBENCLAMIDE IN REDUCING BLOOD GLUCOSE LEVELS OF GLUCOSE - INDUCED HYPERGLYCEMIA IN RATS

ABSTRACT: The objectives of the study are to determine the lethal dose (LD50) of Euphorbia hirta L. lyophilized extract on Sprague- Dawley rats using OECD 420 (Fixed Dose Procedure). Second, the researchers describe the acute toxidrome of Euphobia hirta L.(Tawa-Tawa) based on the changes in central nervous, respiratory, eyes, ears behavior and weight. Third, is to describe the gross and histolopathologic changes in Sprague Dawley rats in 3 days brought about by Euphorbia hirta L. The study used the Fixed Dose Procedure and Quasi-Experimental design. The leaves, stem, flower were subjected to decoction, a process where in the leaves where extracted by boiling. After decoction, it was lyophilized to obtain powdered form of the water-soluble components. The subjects were randomly chosen, one rat was used for the sighting study and five rats were used for the main study. The dose for the sighting study was 300mg/kg body weight and used 2000mg/kg body weight for the main study. The lyophilized Tawa-Tawa extract was tested on laboratory experimental rats and was given as a single dose via lavage. There were no incidence (0%) central nervous depression, on the other hand there were 100% increase in the respiratory rate and startle response, only 20% exhibited fine and course body tremors. Furthermore, only 20 % exhibited changes in the eyes, there were no significant findings in the ears, in autonomic changes, 20 % exhibited salivation and 100 % exhibited tail lashing, pilomotor erection and increase body temperature. For behavioural changes 100 % exhibited passivity and there were also an increased body weight with an average of 0.6 grams. The researchers conclude that the LD50 of Euphorbia hirta L. (tawa-Tawa) is greater than 2000 mg/kg/bw base on OECD 420. The extract has no significant toxic effect on the central nervous system, respiratory system, autonomic nervous system, general appearance of the eyes & ears, body weight and behaviour of Sprague Dawley rats. Also, the researchers conclude that there are no gross abnormality in the organs of Sprague Dawley rats including, heart, lungs, liver, kidneys, spleen, as well as in the small and large intestines. Lastly, the histopathologic results are not sufficient to conclude that there is no toxicity at a dose of 2000 mg/kg/bodyweight due to poor specimen quality.

Department of Pharmacology

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