Caulerpa Lentillifera as a Culture Medium for the Growth of Escherichia Coli, Staphylococcus Aureus, Non-Typhoidal Salmonella Enterica and Pseudomonas Aeruginosa / Charissa Joyce Abeleda ; Trixia Lyn Aboy ; Anna Pamela Agnote ; Meg Angelie Alas ; Alnea Marie Altre ; Charise Leah Arcenas ; Jane Basco ; Glaiza Elaine Dela Peña ; Arielle Dellosa ; Laly Ilagan and Lou Abigail Tandog.

By:

Contributor(s):

Publication details: Fairview, Quezon City School of Medical technology, FEU-NRMF, 2011Description: 72 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2011 0003

Summary: Abstract: Many studies on bacteria and yeasts are being conducted on the whole world. These studies used the most efficient culture media of red algae. These media are manufactured in commercial laboratories which is the reason why they are very complicated and expensive. The main objective of this study was to prepare a culture medium from caulerpa lentillifera in order to support the growth of non-fastidious bacteria such as escherichia coli, staphylococcus, non-typhoidal salmonella enteric and pseudomonas aeruginosa at a cheaper cost. Two forms of broth media were prepared from the caulerpa lentillifera: one prepared from extract and the other from powdered form. The control used in this study was tryptic soy broth medium which is the standard broth medium for the cultivation of different organism. In the broth medium prepared from pure extract, all sub cultured plates had growth up to the 4th quadrant indicating 100% of 4+ growth in all preparations for escherichia coli and pseudomonas aeruginosa. Only one of the three sub cultures from c. Lentillifera inoculated with staphylococcus aureus had growth up to the 4th quardrant of the plate giving a 33.33% 0f growth. In non-typhoidal salmonella enteric, 66.67% or 2 out of 3 tubes had 4+ growth in c. Lentillifera preparation. The identify of each organism used was confirmed using biochemical tests. Cost analysis showed that the broth media from c. Lentillifera is cost-effective compared to the commercially prepared media since it is at least 25% lower than the price of the commercially prepared media in tubes. The computed value for c. Lentillifera is po.36/tube while for the tryptic soy broth is po.81/tube.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Call number	Status	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2011 0003 c.1 (Browse shelf(Opens below))	Available	T001350
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2011 0003 c.2 (Browse shelf(Opens below))	Available	T001351
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2011 0003 c.3 (Browse shelf(Opens below))	Available	T001352

Browsing Far Eastern University - Nicanor Reyes Medical Foundation shelves, Shelving location: Theses Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	MT 2011 0001 c.2 Commercial Cane Vinegar as Diluting Fluid in the Leukocyte Counting Procedure /	MT 2011 0002 c.1 Identification of Bioluminescent Bacteria from Marine Animals Readily Available in the Market /	MT 2011 0002 c.2 Identification of Bioluminescent Bacteria from Marine Animals Readily Available in the Market /	MT 2011 0003 c.1 Caulerpa Lentillifera as a Culture Medium for the Growth of Escherichia Coli, Staphylococcus Aureus, Non-Typhoidal Salmonella Enterica and Pseudomonas Aeruginosa /	MT 2011 0003 c.2 Caulerpa Lentillifera as a Culture Medium for the Growth of Escherichia Coli, Staphylococcus Aureus, Non-Typhoidal Salmonella Enterica and Pseudomonas Aeruginosa /	MT 2011 0003 c.3 Caulerpa Lentillifera as a Culture Medium for the Growth of Escherichia Coli, Staphylococcus Aureus, Non-Typhoidal Salmonella Enterica and Pseudomonas Aeruginosa /	MT 2012 0001 A Tracer Study of the Medical Technology Graduates of Far Eastern University-Dr. Nicanor Reyes Medical Foundation /	Next

Includes appendices and bibliographical references.

Abstract: Many studies on bacteria and yeasts are being conducted on the whole world. These studies used the most efficient culture media of red algae. These media are manufactured in commercial laboratories which is the reason why they are very complicated and expensive. The main objective of this study was to prepare a culture medium from caulerpa lentillifera in order to support the growth of non-fastidious bacteria such as escherichia coli, staphylococcus, non-typhoidal salmonella enteric and pseudomonas aeruginosa at a cheaper cost. Two forms of broth media were prepared from the caulerpa lentillifera: one prepared from extract and the other from powdered form. The control used in this study was tryptic soy broth medium which is the standard broth medium for the cultivation of different organism. In the broth medium prepared from pure extract, all sub cultured plates had growth up to the 4th quadrant indicating 100% of 4+ growth in all preparations for escherichia coli and pseudomonas aeruginosa. Only one of the three sub cultures from c. Lentillifera inoculated with staphylococcus aureus had growth up to the 4th quardrant of the plate giving a 33.33% 0f growth. In non-typhoidal salmonella enteric, 66.67% or 2 out of 3 tubes had 4+ growth in c. Lentillifera preparation. The identify of each organism used was confirmed using biochemical tests. Cost analysis showed that the broth media from c. Lentillifera is cost-effective compared to the commercially prepared media since it is at least 25% lower than the price of the commercially prepared media in tubes. The computed value for c. Lentillifera is po.36/tube while for the tryptic soy broth is po.81/tube.

Thesis - School of Medical Technology

There are no comments on this title.

to post a comment.