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The Effect of 5% Acetic on the Visualization of Bloody Cervicovaginal Smear Stained with Papanicolau / Francis Christian P. Aquiler ; Song Seob Kim ; Emory V. Macalacad ; Molly Audrey P. Motos ; JoyAleine Najarro ; Roxanne Ingrid Y. Ngo ; Emmanuel John R. Pascua ; Dainel Edgar A. Reyes and Jasmine Saguinsin.

By: Contributor(s): Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF August, 2012Description: 68 pages illustrations, tables, photos; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
LOC classification:
  • MT 2012 0004
Summary: Abstract: Cervical cancer is the third most common type of malignancy among females worldwide and early detection of it has been associated with decreased mortality. Cervicovaginal smear, more commonly known as Pap smear, has been the traditional screening procedure for diagnosing abnormal changes found in the uterine cervix. However, cervicovaginal smear is not free from hindraces which may interfere in the interpretation of a pathologist.some examples of these hindrances are dying artifacts and excessive blood. A bloody cervicovaginal smear leads to specimens which are unsatisfactory for evaluation and taken back to the OB GYNE for repeat collection. Cervical cancer often presents with abnormal uterine bleeding, thus a bloody specimen may always be inevitable. With this problem in hand, the researchers used 5% Acetic acid as an intervention in a conventional Pap smear procedure which lyses the red blood cells and allow the visualization of the significant cells. Twelve patients who presented with abnormal uterine bleeding were selected as participants in this study. Two specimens were collected from each patient; one was subjected to the intervention of 5% acetic acid and the other one served as control for comparison. Both specimen will then undergo the conventional Pap smear procedure and taken to a pathologist fo evaluation of visualization. The visualization of each specimen was collected. For the control, 10 or 83.3% of the specimens were categorized as not visualized and 2 or 16.7% were categorized as partially visualized while on the intervention of 5% acetic acid, all or 100% of the specimens were categorized as visualized. This study used Wilcoxon Signed Rank Test to compare the visualization. Having a computed p-value of 0.001 and a level of significance of 0.05 the visualization between the control and intervention of 5% acetic acid has a significant difference. Hence, lysing red blood cells on a bloody cervicovaginal smear would make the significant cells visualized thus a more suitable specimen for evaluation.
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Item type Current library Call number Status Date due Barcode
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2012 0004 (Browse shelf(Opens below)) Available T001137
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2012 0004 c.2 (Browse shelf(Opens below)) Available T001323

Includes appendices and bibliographical references.

Abstract: Cervical cancer is the third most common type of malignancy among females worldwide and early detection of it has been associated with decreased mortality. Cervicovaginal smear, more commonly known as Pap smear, has been the traditional screening procedure for diagnosing abnormal changes found in the uterine cervix. However, cervicovaginal smear is not free from hindraces which may interfere in the interpretation of a pathologist.some examples of these hindrances are dying artifacts and excessive blood. A bloody cervicovaginal smear leads to specimens which are unsatisfactory for evaluation and taken back to the OB GYNE for repeat collection. Cervical cancer often presents with abnormal uterine bleeding, thus a bloody specimen may always be inevitable. With this problem in hand, the researchers used 5% Acetic acid as an intervention in a conventional Pap smear procedure which lyses the red blood cells and allow the visualization of the significant cells. Twelve patients who presented with abnormal uterine bleeding were selected as participants in this study. Two specimens were collected from each patient; one was subjected to the intervention of 5% acetic acid and the other one served as control for comparison. Both specimen will then undergo the conventional Pap smear procedure and taken to a pathologist fo evaluation of visualization. The visualization of each specimen was collected. For the control, 10 or 83.3% of the specimens were categorized as not visualized and 2 or 16.7% were categorized as partially visualized while on the intervention of 5% acetic acid, all or 100% of the specimens were categorized as visualized. This study used Wilcoxon Signed Rank Test to compare the visualization. Having a computed p-value of 0.001 and a level of significance of 0.05 the visualization between the control and intervention of 5% acetic acid has a significant difference. Hence, lysing red blood cells on a bloody cervicovaginal smear would make the significant cells visualized thus a more suitable specimen for evaluation.

Thesis - School of Medical Technology

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