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A Comparative Study of Coconut Water to Hayem's Solution as Diluting Fluid in RBC Counting Procedure / Irvine Kate Q. Abne ; Allena Jan S. Abulencia ; Raphael Paul C. Cadiz ; Erika Lou C. Curata ; Maikee Bernadine S. Halili ; Kathryne F Lozano ; Micah V. Pangilinan ; Clerysa Pauline P. San Felipe and Eolus Lemuel V. Vilela.

By: Contributor(s): Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF October 2014Description: 38 pages: illustrations, tables, photos; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
LOC classification:
  • MT 2014 0007
Summary: Abstract: Red Blood Cell Count using Coconut Water as Alternative Diluting Fluid. Red blood cell (RBC) count is always performed as part of a complete blood cell test to diagnose anomalies in the body involving the red cells of certain individual. This could also help us in determining the well being and status of a patient. Complete blood count being the most requested procedure in clinical laboratory is performed to determine whether a person has blood anomaly such as anemia, leukemia, thalassemia, and certain abnormalities in the red cells like poikolocytosis and anisocytosis. Procedure on RBC counting uses isotonic solutions for dilution to avoid RBCs from being ruptured due to osmotic pressure. Tonicity of the coconut water was comparable that of the standard diluting fluid used in RBC counting thus this was used as an alternative for Hayem's fluid as the standard reagent used for the manual red cell counting. Also considering the fact that the coconut water is readily available, cheap, sterile, pyrogen-free, non-antigenic and non-hemolysing in human cells, it is also a natural isotonic beverage with the same level of electrolytic balance as we have in our blood and also boost poor blood circulation. To find out the answer to our research problem, twenty (20) blood samples were extracted from the volunteer qualified blood donors and manual RBC count was performed. Both coconut water and hayem's solution were used as diluting fluid for each sample and results were compared. After computing for the significant difference of each result using T-test, we have found that coconut water can't be used as an alternative diluting fluid for RBC counting. Furthermore, repeat RBC count testing must be done to ensure accurate results.
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Item type Current library Call number Status Date due Barcode
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2014 0007 c.3 (Browse shelf(Opens below)) Available T001363
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2014 0007 c.4 (Browse shelf(Opens below)) Available T001364
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2014 0007 c.2 (Browse shelf(Opens below)) Available T001362
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2014 0007 c.1 (Browse shelf(Opens below)) Available T001361

Includes appendices and bibliographical references.

Abstract: Red Blood Cell Count using Coconut Water as Alternative Diluting Fluid. Red blood cell (RBC) count is always performed as part of a complete blood cell test to diagnose anomalies in the body involving the red cells of certain individual. This could also help us in determining the well being and status of a patient. Complete blood count being the most requested procedure in clinical laboratory is performed to determine whether a person has blood anomaly such as anemia, leukemia, thalassemia, and certain abnormalities in the red cells like poikolocytosis and anisocytosis. Procedure on RBC counting uses isotonic solutions for dilution to avoid RBCs from being ruptured due to osmotic pressure. Tonicity of the coconut water was comparable that of the standard diluting fluid used in RBC counting thus this was used as an alternative for Hayem's fluid as the standard reagent used for the manual red cell counting. Also considering the fact that the coconut water is readily available, cheap, sterile, pyrogen-free, non-antigenic and non-hemolysing in human cells, it is also a natural isotonic beverage with the same level of electrolytic balance as we have in our blood and also boost poor blood circulation. To find out the answer to our research problem, twenty (20) blood samples were extracted from the volunteer qualified blood donors and manual RBC count was performed. Both coconut water and hayem's solution were used as diluting fluid for each sample and results were compared. After computing for the significant difference of each result using T-test, we have found that coconut water can't be used as an alternative diluting fluid for RBC counting. Furthermore, repeat RBC count testing must be done to ensure accurate results.

Thesis - School of Medical Technology

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