Biofilm Inhibition by Piper Betle Leaf Essential Oil Extract Against Pseudomonas Aeruginosa / John Vincent Costes ; Aemarr Victor Ernest Seares Corpus ; Martin John G. Gaspi ; Mary Grace Labayen ; Kharla Pabustan ; Frances Alyssa Pena ; Maria Cielo Tamayo and Nigel Tayone.

Includes appendices and bibliographical references.

Abstract: The study evaluates the efficiency of the Piper betel Leaf Essential Oil Extract against the organism Pseudomonas aeruginosa and its virulence factors, specially biofilm formation through testing by using of Broth Macro4dilution. The study also evaluates the minimum inhibitory concentration and effectivity of the plant's essential oil extract to inhibit biofilm formation. The results of the study revealed that pseudomonas aeruginosa and its biofilm is best evaluated through tube macrodilution. Test show that its inhibitory concentration ranges between 62.5 ul and above of the oils concentration suggesting that the concentration is enough to inhibit the virulence factor of biofilm formation from happening. The test was ran two times each with the same amount of the oil and the organism. This was done to prove the accuracy and precision of the test. In conclusion, the Piper Betle Leaf Essential Oil Extract is effective in inhibiting the biofilm formation through the inhibition of the bacteria's communication but only in a certain concentration. The researchers made their recommendations to further develop the study.

Thesis - School of Medical Technology