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The Use of Brassica Oleracea var. Capitata L.F. Rubra Family Brassiaceae (Red Cabbage) Extract in Serum Albumin Determination / Princess Marie I. Broñola ; Jerome P. Carreon ; Kevin Michael Y. De Castro ; Krys Anette P. Ferreria ; Mary Coleen DR. Ganiron ; Danya C. Gutierrez ; Ross Bennie C. Herrera ; Milligems C. Lappay ; Nica Jeline A. Macaraeg and Nicole Anne B. San Diego.

By: Contributor(s): Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF June 2017Description: 79 pages: illustrations, tables, photos; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
LOC classification:
  • MT 2017 0001
Summary: Abstract: Protein determination levels are requested for those patients suffering with disease associated with an increased or decreased serum albumin levels. The usual test performed for detecting human serum albumin levels in Bromocresol green dye-binding method. Bromocresol green is pH indicator which produces a specific color change at a certain pH. The reaction for dye-binding method is based on the affinity of human serum albumin with BCG causing the absorbance which is proportional to the albumin concentration. On the other hand, anthocyanins are plant pigments discovered to have a array of beneficial effects in the human health. They also produce certain colors at various pH ranges, hence they can be used as pH indicators. In case of red cabbage, it is said to contain anthocyanin and produces color changes in acidic and basic environment. This research used the ethanolic crude extract of red cabbage in comparison for BCG in its effectiveness in detecting human serum albumin. Ethanolic extraction of red cabbage had been prepared by rotary evaporation. The sample were obtained by accidental haphazard random used by Far Easter University-Nicanor Reyes Medical Foundation's laboratory. The test was performed using spectrophotometric method similar to BCG method that used 1.5 ml red cabbage crude extract and 0.01 ml serum. The result using red cabbage crude extract showed that it was not effective as BCG. One of the major factors which had cause its effectiveness in binding human serum albumin was that, the researchers only used a crude extract of red cabbage in which can't quantify the amount of anthocyanin present.
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Item type Current library Call number Status Date due Barcode
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2017 0001 c.2 (Browse shelf(Opens below)) Available T001671
Theses Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2017 0001 (Browse shelf(Opens below)) Available T001658

Includes appendices and bibliographical references.

Abstract: Protein determination levels are requested for those patients suffering with disease associated with an increased or decreased serum albumin levels. The usual test performed for detecting human serum albumin levels in Bromocresol green dye-binding method. Bromocresol green is pH indicator which produces a specific color change at a certain pH. The reaction for dye-binding method is based on the affinity of human serum albumin with BCG causing the absorbance which is proportional to the albumin concentration. On the other hand, anthocyanins are plant pigments discovered to have a array of beneficial effects in the human health. They also produce certain colors at various pH ranges, hence they can be used as pH indicators. In case of red cabbage, it is said to contain anthocyanin and produces color changes in acidic and basic environment. This research used the ethanolic crude extract of red cabbage in comparison for BCG in its effectiveness in detecting human serum albumin. Ethanolic extraction of red cabbage had been prepared by rotary evaporation. The sample were obtained by accidental haphazard random used by Far Easter University-Nicanor Reyes Medical Foundation's laboratory. The test was performed using spectrophotometric method similar to BCG method that used 1.5 ml red cabbage crude extract and 0.01 ml serum. The result using red cabbage crude extract showed that it was not effective as BCG. One of the major factors which had cause its effectiveness in binding human serum albumin was that, the researchers only used a crude extract of red cabbage in which can't quantify the amount of anthocyanin present.

School of Medical Technology

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