Commercially-Prepared Ascorbic Acid as Diluting Fluid for Manual WBC Count / Sheila S. Bermido ; Caryl Anne Monique E. Bianzon ; Janrev A. Cordova ; Eduardo Marco V. Dayrit ; Marielle D. Lacsamana ; Eloisa Rachel C. Lobo and Jacob R. Ragasa.

By:

Sherwin N. Reyes, RMT, MSc, ISID [research committee]

Contributor(s):

Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF 2016Description: 62 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2016 0009

Summary: Abstract: Due to the potential harmful effects of the glacial acetic acid, a commonly used diluting fluid for manual WBC count, the researchers proposed to used commercially-prepared ascorbic acid to lyse the red blood cells while maintaining the morphology of the white blood cells. This study aims to compare the capability of commercially- prepared ascorbic acid as a lysing agent in a diluting fluid to glacial acetic acid. Specifically, this study aims to compare their capabilities in lysing red blood cells, maintaining white blood cell morphology and white blood cell count. For the preparation of the commercially-prepared ascorbic acid diluting fluid, the ground ascorbic acid tablets were diluted with distilled water. The ascorbic acid solution was then mixed with methylene blue as a straining agent, and the routine procedures for manual WBC count followed. To compare the capability of commercially-prepared ascorbic acid to acid as the diluting fluid.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2016 0009 (Browse shelf(Opens below))	Available		T001239

Includes appendices and bibliographical reference.

Abstract: Due to the potential harmful effects of the glacial acetic acid, a commonly used diluting fluid for manual WBC count, the researchers proposed to used commercially-prepared ascorbic acid to lyse the red blood cells while maintaining the morphology of the white blood cells. This study aims to compare the capability of commercially- prepared ascorbic acid as a lysing agent in a diluting fluid to glacial acetic acid. Specifically, this study aims to compare their capabilities in lysing red blood cells, maintaining white blood cell morphology and white blood cell count. For the preparation of the commercially-prepared ascorbic acid diluting fluid, the ground ascorbic acid tablets were diluted with distilled water. The ascorbic acid solution was then mixed with methylene blue as a straining agent, and the routine procedures for manual WBC count followed. To compare the capability of commercially-prepared ascorbic acid to acid as the diluting fluid.

Thesis - School of Medical Technology

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