A PRELIMINARY STUDY ON THE COMPARISON OF TSAANG GUBAT (CARMONA RETUSA) AND LOPERAMIDE ON ITS EFFECTS ON GI MOTILITY OF DUODENAL SEGMENTS OF MALE RABBITS

ABSTRACT: This study aims to compare the anti-motility effect of Lopperamide and Tsaang gubat (Carmona retusa) and to determine at which dose of Tsaang gubat (Carmona retusa) will have a comparable effect on the rate and amplitude of duodenal contraction of 5 male rabbits. In this experimental study, a 100% stock solution of Tsaang gubat was prepared using the decoction method of extraction. From this solution, a 6g/kg 7g/kg, 8g/kg, 9g/kg, 10g/kg, and 11g/kg dosage of Tsaang gubat was prepared. The Loperamide solution was prepared using Loperamide capsules (2mg). In the experimental procedure, Tyrode solution was used to maintain viability of intestinal segments. Also 5 male rabbits of around 7kg - 1.5kg body weight were used. The data to be collected from the Kymograph was the rate and amplitude of duodenal contractions. Only one concentration of Loperamide was used for the entire experimental. Following its administration, six different dosages of Tsaang gubat were administered one after the other. Each of the drugs administered was allowed to run for 5 minutes. Based on the results obtained in the experiment, the researchers concluded that increasing the dose of Tsaang gubat would increase the amplitude while decreasing the rate of intestinal contractions. They also stated that Tsaang gubat has a comparable anti-motility effect (rate and amplitude) with Loperamide at a dose of around 7g/kg

Department of Pharmacology