A STUDY OF MOMORDICA CHARANTIA (AMPALAYA) EXTRACT ON THE DOSE AND ITS PROPORTION IN LOWERING BLOOD GLUCOSE LEVEL OF HYPERGLYCEMIC MICE

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Language: English Publication details: Quezon City FEU-NRMF March 2003Description: 42 pagesLOC classification:

MPHAR20030016

Summary: ABSTRACT: The Momordica charantia (Ccucurbitaceae) leaves were collected and air-dried for two weeks. They were grinded using an ordinary blender until powdery in texture. Extraction procedures were done using 80% ethyl alcohol. The percolate was collected and filtered. Finally, it was exposed to room temperature until a syrupy consistency was obtained. The Ampalaya extract was dissolved in distilled water in a 1:1 proportion to obtain a 100% stock solution. Different proportion or dilution were made from the crude extract namely 1:20, 1:10 and 3:20. Likewise, different doses based on the weight of mice were computed using 0.52 log interval such as 20mg/kg, 66mg/kg, 280mg/kg respectively. 36 healthy randomized and properly identified adult male and female swiss mice were used. Each was kept in individual cages. They were group into four; 9 swiss mice per group. Swiss mice were subgrouped into three, 3 mice per dose level. The animals were fasted for food and water for 12 hours before the test. Baseline blood sugar levels (BSL) were determined for all animals by nipping tail end and were recorded as the fasting blood sugar. Hyperglycemia was induced by intraperitoneal injection of 50% glucose solution at 165mg/kg body weight. Blood sugar levels after 1 hour was taken as the hyperglycemic state. Then, different dilutions of ampalaya extract were administered intraperitoneally to the three groups of mice. NSS was injected intraperitoneally to the fourth group, which is the control group. Blood sugar levels were determined 1 hr, 2 hrs, 3 hrs after administration of extract/NSS. Biostatical analysis using T-test analysis and Manova were conducted to determine the hypoglycemic effects using swiss mice through parameters such as dose levels and proportion. The test showed significant hypoglycemic effect at 1:20 proprtion. The blood glucose levels do not depend on dose consideration. Its activity was compared to normal saline solution (NSS) as the control.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MPHAR20030016c.2 (Browse shelf(Opens below))	Available		T001792
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MPHAR20030016c.1 (Browse shelf(Opens below))	Available		T000886

ABSTRACT: The Momordica charantia (Ccucurbitaceae) leaves were collected and air-dried for two weeks. They were grinded using an ordinary blender until powdery in texture. Extraction procedures were done using 80% ethyl alcohol. The percolate was collected and filtered. Finally, it was exposed to room temperature until a syrupy consistency was obtained. The Ampalaya extract was dissolved in distilled water in a 1:1 proportion to obtain a 100% stock solution. Different proportion or dilution were made from the crude extract namely 1:20, 1:10 and 3:20. Likewise, different doses based on the weight of mice were computed using 0.52 log interval such as 20mg/kg, 66mg/kg, 280mg/kg respectively. 36 healthy randomized and properly identified adult male and female swiss mice were used. Each was kept in individual cages. They were group into four; 9 swiss mice per group. Swiss mice were subgrouped into three, 3 mice per dose level. The animals were fasted for food and water for 12 hours before the test. Baseline blood sugar levels (BSL) were determined for all animals by nipping tail end and were recorded as the fasting blood sugar. Hyperglycemia was induced by intraperitoneal injection of 50% glucose solution at 165mg/kg body weight. Blood sugar levels after 1 hour was taken as the hyperglycemic state. Then, different dilutions of ampalaya extract were administered intraperitoneally to the three groups of mice. NSS was injected intraperitoneally to the fourth group, which is the control group. Blood sugar levels were determined 1 hr, 2 hrs, 3 hrs after administration of extract/NSS. Biostatical analysis using T-test analysis and Manova were conducted to determine the hypoglycemic effects using swiss mice through parameters such as dose levels and proportion. The test showed significant hypoglycemic effect at 1:20 proprtion. The blood glucose levels do not depend on dose consideration. Its activity was compared to normal saline solution (NSS) as the control.

Department of Pharmacology

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