Antimicrobial effect of the crude methanol extracts of three marine sponges from Bolinao, Pangasinan on selected pathogens using agar diffusion method (August-September, 1993) / Rowena P. Cardenas, Cristina U. Chua, Rachelle U. Chua, Lorichelle B. Dela Cruz, Niel Andrew S.J. De Lumen, Gladys S. Demdam, Ann Veronique C. Eguaras, Katherine T. Gabon, Anthony G. Jao , Ann Rizza B. Justimbaste, Raquel B. Laylo, Glenn M. Luzano, Zarah S. Malo, Fe F. Mangalindan, Jeanne U. Ngo, Gjay L. Ordinal, Cherry C. Padilla, Claudette P. Ricero, Stella Marie S. Rondilla and Joselito R. Yulo.

Includes appendices and bibliographical references.

Abstract: The oceans are a largely entapped reservoir for interesting, unusual and potentially useful natural pharmaceutical products (fox, 1982). The potentials of our local marine sponges as a source of drugs or as models for the synthesis of isolated and identified active compounds show promising results. It is then the objective of this in vitro study to determine the antimicrobial activity of the crusade methanol extract of three unknown marine sponges coded : S28, S50 S50A from Bolinao, Pangasinan on selected pathogens : E. coli, S. typhi, P. aeruginosa, P. mirabilis, Enterobacter sp. using Agar Diffusion Method by measuring their zones of inhibitions and comparing them to those exhibited by known antibiotics : Chloramphenicol for S. typhi and Norfloxacin for the rest of the organisms. Results revealed that all three sponge extracts exhibited significant antimicrobial activity against S. typhi and P. aeruginosa, two extracts (S50 and S50A) against P. mirabilis, and S28 against Enterobacter sp. Growth of E. coli was not at all arrested by any of the sponges, This suggest that the aforementioned sponges can be at par with our known antibiotics should further studies be pursued requiring isolation and characterization of each identifiable active compound.

Thesis - Department of Community & Family Medicine