Hemagglutinating Capacity of the Crude Extract of Phaseolus Lunatus (Fam. Fabaceae) in Abo Typing / Florencio L. Alfonso, IV ; Sergielle F. Aquino ; Aldanna Felicci G. Carag ; Ivy Joan C. Dela Cruz ; Eleiza Marie D.C. Garcia ; Andrea Beatriz U. Gonzales ; Jules Dianne B. Mariano ; Therese Nadugo ; Emmanuelle John P. Palmere ; Jorena C. Reburiano ; Vincent William C. Reyes and John Nikolai T. Teves.

Includes appendices and bibliographical references.

Abstract: This research aimed to determine the ability of the crude extract of Phaseolus lunatus (Lima beans) to agglutinate blood types and to what specific blood type will it agglutinate. The crude extract of the Phaseolus lunatus were extracted through simple manual extraction and was diluted to different concentrations using the Normal Saline Solution (NSS) as the diluent. Different concentrations of the Phaseolus lunatus crude extract were used to be able to determine the minimum concentration that agglutinate different blood types. The blood samples extracted from donors with blood type of A, B, AB, and Ounderwent RBC suspension and were used for blood typing. The donors' blood samples used came from the discarded EDTA tubes from the blood bank of a laboratory. The blood typing included the use of the crude extract from Phaseolus lunatus and reagents, Anti-A and Anti-B, as the positive and negative controls. The results obtained from the test were evaluated by three licensed medical technologist and graded them according to the degree of agglutination. There were 20 screened and decoded blood samples that were tested according to their respective blood groups. Phaseolus lunatus positively agglutinated four out of five or 80% of the blood type "A" in concentrated Lima bean crude extract (T0), in 0.9 mL of Lima bean extract diluted with 0.1 mL of NSS (T1) and with 0.7 mL of Lima extract diluted with 0.3 mL of NSS (T3) while 0.8 mL of Lima bean extract that is diluted with 0.2 mL of NSS (T2) positively agglutinated all 5 (100%) blood samples. Blood type "A" has the highest percentage of positive agglutination followed by the blood type "AB" which score three out of five or 60% positive agglutination at concentration T2. Both T0 and T1 resulted to all five (100%) blood samples being agglutinated four out of five or 80% blood samples. On the other hand, only two out of five or 20%of the blood type "B" showed positive agglutination at concentrations T1 and T2. Then all five (100%) blood type "B" samples showed no agglutination when combined with T3. Lastly, blood type "O" yielded five out of five or 100% negative result in all concentrations. The crude extract of Phaseolus lunatus was capable of agglutinating all blood types except for blood type "O". Blood type "A" showed the highest percentage of positive results and suggested that lectins present in this crude extract acts similarly with Anti-sera A. However, these impurities from the crude extract may have caused the negative reactions to blood type "A" and blood type "AB". It has been ruled out that there are no minimum concentrations to determine the hemagglutinating capacity of the crude extract of Phaseolus, since blood type "A" , "B" and "AB" showed inconsistent agglutination when combined with the different concentrations.

Thesis - School of Medical Technology