Brown Rice as an Enriched Substance for Culture Medium of Trichophyton Rubrum / Annabelle N. Austria ; Alyssa Elaine Q. Banzon ; Romena Liza C. Fernandez ; Reyzelle Ann D. Logramonte ; Jolly Micah V. Magsino ; Marc Brendon T. Mamporte ; Eun Chong Meang ; Eun Hea Meang ; Rusela Maridith S. Nuñez ; Krysthia Isobel M. Perez and Jeric Allan B. Tan.

Includes appendices and bibliographical references.

Abstract: Brown rice is unpolished whole grain rice that is produced by removing only the hull or husk using a mortar and pestle or rubber rolls. It may be distinctly brown, reddish or purplish. Unknown to many, the bran layer contains very important such as rhiamine and significant quantities of starch, which Trchophyton rubrum needs for growth. With this, the preparation of brown rice as enrichment substance cheaper costs than preparation of Sabouraud`s Dextrose Agar, American Bacteriologic Agar and Mycobiotic Agar which are chemically synthetic media or culturing fungi. Thus, an experimental study of Brown rice as an enrichment substance was carried out in determining the growth of Trichophyton rubrum. Results of the study conducted at the Microbiology Laboratory of Far Eastern University- Dr. Nicanor Reyes Medical Foundation, SDA with 5g and 10g of brown rice have 100% growth of Trichophyton rubrum in all of the tube media, while 80% of growth of Trichophyton rubrum in American Bacteriologic Agar and 5g brown rice (ABA) was seen, and only 50% growth of Trichophyton rubrum in 10g brown rice (ABA). On other hand, there`s growth of Trichophyton rubrum on 100% Brown Rice 5g with Mycobiotic agar and only 60% in Brown Rice 10g with Mycobiotic Agar. Macroscopic characteristic of Trichophyton rubrum were the same on all of the media, which have a colonial morphology seen as flat or heaped-up colony that is generally white to reddish with a cottony or velvety surface and also all of the inoculated media with Trichophyton rubrum have the same microscopic morphology which is tear-drop shaped microconidia. The lesser concentration of brown rice shows more abundant growth of Trichophyton rubrum. Therefore, brown rice can be used as an enrichment substance for culture media of Trichophyton rubrum.

Thesis - School of Medical Technology