Coconut oil and palm oil as substitute cleaning agents of Xylene in tissue processing / Althea M. Bacayana ; Bettina Anne Louise I. Colobong ; Lyssa Mae E. De Belen ; Danzelle Macrine B. Collantes ; Marielle Coleen P. Domingo ; Jas Merain J. Fabello ; Glizel B. Maga ; Lou Modesselle W. Mandocdoc ; Joseph Angelo B. Racelis ; Marielle Erika C. Radin and Nathanielle Mae U. Rosal.

Contributor(s):

Language: english Publication details: Fairview, Quezon City: School of Medical Technology, FEU-NRMF, 2019.Description: 55 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2019 0008

Summary: Abstract: Xylene is the most commonly used clearing agent in tissue processing that can be toxic and carcinogenic especially in direct contact or long term exposure. The researchers aim to determine whether coconut oil and palm oil are effective substitutes for xylene as clearing agents in routine histhopathologic procedure. Twelve samples of pork tissue were divided into three experimental groups labelled A, B and C. The researchers used various organs of pig tissue specifically: lungs, large intestine, skeletal muscle and liver obtained from a local market. Group A was Cleared with xylene and group B and C with coconut oil and palm oil respectively. The tissue sections were then stained using hematoxylin and eosin (H&E stain). The results indicated that coconut oil and palm oil showed no significant difference with that of xylene thus exhibiting comparable clearing and staining capacity.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2019 0008 c.1 (Browse shelf(Opens below))	Available		T002062
Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2019 0008 c.2 (Browse shelf(Opens below))	Available		T002063

Includes appendices and bibliographical references.

Abstract: Xylene is the most commonly used clearing agent in tissue processing that can be toxic and carcinogenic especially in direct contact or long term exposure. The researchers aim to determine whether coconut oil and palm oil are effective substitutes for xylene as clearing agents in routine histhopathologic procedure. Twelve samples of pork tissue were divided into three experimental groups labelled A, B and C. The researchers used various organs of pig tissue specifically: lungs, large intestine, skeletal muscle and liver obtained from a local market. Group A was Cleared with xylene and group B and C with coconut oil and palm oil respectively. The tissue sections were then stained using hematoxylin and eosin (H&E stain). The results indicated that coconut oil and palm oil showed no significant difference with that of xylene thus exhibiting comparable clearing and staining capacity.

Thesis - School of Medical Technology

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