Comparison of Leaf, Pell, and Seed Extract of Citrus Aurantifolia as Larvicidal Agent as Aedes Aegypti / Josephine Armanne D. Aluyen ; Paul Laurence A. Del Rosari ; Monica Ysabella S. Domingo ; Glenn Nicole M. Esposo ; | Ferisa Nene C. Garcia ; Anthon Migg C. Ico; Rina L. Kabayama ; Amiel Ced C. Malilay; Jeremy Carlos M. Montinola ; Lennart John L. Petallana and Owens Christiene Therese C. Tabujara.

Includes appendices and bibliographical references.

Abstract: The researchers would like investigate on which part the 'Citrus aurantifolia' (namely and limited to the leaf, peel, and seed) when expressed as an extract can be a potential and effective larvicide against 'Aedes aegypti' the main vector of Dengue in the Philippines. The ongoing risk of the said disease is increasing twenty-fold and perhaps will still increase if not stopped or prevent. Numerous researches have been conducted using citrus fruit extracts since they contain limonoid compounds which said to have the capability to be cytotoxic and kill the developing larva. By getting the significant differences of each part extract, the researches may be able to quantitively evaluate and compare which part is the most potent. The method was adapted from the WHO larvicidal bioassay and the data shall be treated using the One-Way ANOVA (Analysis of Variance) and Multiple Comparisons Testing. Furthermore, a qualitative study was also done by comparing the morphology of a representative dead moribund larva from the extract that killed most of the sample larvae to adead moribund larva of the positive control. The researches may be able to conclude that there is a statistical difference between the three extracts F(12,75) = 6.682 p-value = 0.002. A series of multiple comparisons tests were done, one of which is the Post Hoc Turkey test. It revealed that the peel extract has a significant difference between the seed and the and the leaf extract on a 95% confidence interval. Analysis of Covariance which is combination of ANOVA and Estimated Marginal Mean only indicates at the assay done was linear and within accepted error. With regards to the morphological changes undergone by the Aedes aegypti larva 90% Citrus aurantifolia peel extract seen under a dissection microscope, noticeable blackening of the belly and curvature of the body signifies that the organism ws confirmed dead which parallels the changes that have undergone by the moribund larvae of the positive control. However, the researchers recommend a more sophisticated approach when it comes to extracting the essential oil of the subject fruit and also in the method of detecting morphological changes especially the head and the siphon.

Thesis - School of Medical Technology