Citrus microcarpa bunge (family rutaceae)-derived citrate as an alternative laboratoryanticoagulant to commercially-prepared sodium citrate for PTT test / Froilan Conrad P. Bacungan ; Amber Justin O. Chua ; Michelle S. Delos Reyes ; Rashina Christine V. Duran ; Mary Therese Ernestine V. Faller ; Chelsea M. Fernandez ; Patrizia Anne M. Gonzales ; Justin Adrian G. Jardiolin ; Geraldine R. Jazul ; Claire S.P. Mabalot ; Kristine Jan T. Montes and Jeñino Carlo N. Sanchez.

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Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF, 2018Description: 44 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2018 0005

Summary: ABSTRACT: Sodium citrate can function as a buffer, calcium sequestrant, and preservative. The primary aim of sodium citrate in hematology is to prevent coagulation of blood by binding the calcium of blood, specifically plasma, in a soluble complex hence, it is known to be an anticoagulant of choice for coagulation studies. The citrate in this anticoagulant is a derivative of citric acid. Citric acid itself can be an outstanding chelator which can be found in fruits and vegetables. For that reason, this research intended to verify whether Citrus microcarpa-derived citrate will have the same effect when compared to commercially-prepared sodium citrate. To do so, extracted acid from Citrus microcarpa was allowed to crystallize for two (2) weeks to make a final anticoagulant. A sample population of twenty (20) people was randomly selected. Three tubes were reserved for each person-one plain tube (4 ml of blood), one sodium citrate tube (2 ml of blood), and one Citrus microcarpa derived citrate tube (2 ml of blood). Observation of clot formation was also being noted by macroscopic inspection upon transfer, after 5 minutes, and after 30 minutes of transfer for further verification. The blood extracted was then used for testing the efficacy of Citrus microcarpa-derived citrate in automated PTT tests. Result s were tabulated and compared and connote that Citrus microcarpa -derived anticoagulant revealed significant differences as compared to commercially prepared sodium citrate.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2018 0005 c.1 (Browse shelf(Opens below))	Available		T001968
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2018 0005 c.2 (Browse shelf(Opens below))	Available		T001969

Includes appendices and bibliographical references.

ABSTRACT: Sodium citrate can function as a buffer, calcium sequestrant, and preservative. The primary aim of sodium citrate in hematology is to prevent coagulation of blood by binding the calcium of blood, specifically plasma, in a soluble complex hence, it is known to be an anticoagulant of choice for coagulation studies. The citrate in this anticoagulant is a derivative of citric acid. Citric acid itself can be an outstanding chelator which can be found in fruits and vegetables. For that reason, this research intended to verify whether Citrus microcarpa-derived citrate will have the same effect when compared to commercially-prepared sodium citrate. To do so, extracted acid from Citrus microcarpa was allowed to crystallize for two (2) weeks to make a final anticoagulant. A sample population of twenty (20) people was randomly selected. Three tubes were reserved for each person-one plain tube (4 ml of blood), one sodium citrate tube (2 ml of blood), and one Citrus microcarpa derived citrate tube (2 ml of blood). Observation of clot formation was also being noted by macroscopic inspection upon transfer, after 5 minutes, and after 30 minutes of transfer for further verification. The blood extracted was then used for testing the efficacy of Citrus microcarpa-derived citrate in automated PTT tests. Result s were tabulated and compared and connote that Citrus microcarpa -derived anticoagulant revealed significant differences as compared to commercially prepared sodium citrate.

Thesis - School of Medical Technology

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