Anti-Quorum Sensing Property Of Moringa Oleifera Lam, Ethanolic Leaf Extract Against Serratia Marcescens / Claudine Jean C. Abenojar ; Sean Axel Carbonell ; Joyce Anne N. Escalante ; Samantha Alexis S. Ortonio ; Rofly Chelige L. Padilla ; Marjorie Therese H. Sales ; Charlene P. Samson ; Kristoffer Bryan G. Sy ; Sarah Tiffany A. Tangpos and Justin Paul S. Tecson.

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Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF June 2017Description: 48 pages: illustration, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2017 0003

Summary: Abstract: Quorum sensing is a system of stimuli in bacteria used to regulate gene expression in response to fluctuations in cell-population density. This relies upon the interaction of a signal molecule in a bacterial cell with a receptor that specially detects this molecule that is also in the bacteria of the same population. When there is a considerable amount of signal molecules, these will eventually show phenotypic markers such as virulence factors. Bacteria are currently resisting antimicrobials that are available today, it is only a matter of time before the majority of the population suffers from anti-microbial resistance. Anti-quorum sensing is a medium that can utilize natural compounds for the researchers to be able to conclude that certain organic compounds can have a big impact in terms of having a bactericidal or bacteriostatic effect against these organisms. This study utilized Malunggay ( Moringa Oleifera Lam ) ethanolic leaf extract in selectively blocking acylated Homoserine Lactones (AHL) which regulates the expression of phenotypic characters such as prodigiosin, protease, biofilm formation, and c6-ahl which regulates the hemolysin production, thus inhibiting Serratia marcescens quorum sensing. In this study, the researchers determined the anti-quorum sensing capabilities of the said extract by evaluating the pigment production produced by the bacteria. The researchers utilized 2-fold and 10-fold serial dilutions of the extract in their evaluation. The dilutions were used to determine three parameters namely: Minimum Inhibitory Concentration, Pigment Inhibition, and Minimum Bactericidal Concentration. In this experiment, growth has been observed in all of the dilutions performed in the three parameters. This led to the conclusion that Malunggay (Moringa olifera Lam.) ethanolic leaf extract cannot inhibit the pigment produced by Serratia marcescens.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2017 0003 c.2 (Browse shelf(Opens below))	Available		T001673
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2017 0003 (Browse shelf(Opens below))	Available		T001660

Includes appendices and bibliographical references.

Abstract: Quorum sensing is a system of stimuli in bacteria used to regulate gene expression in response to fluctuations in cell-population density. This relies upon the interaction of a signal molecule in a bacterial cell with a receptor that specially detects this molecule that is also in the bacteria of the same population. When there is a considerable amount of signal molecules, these will eventually show phenotypic markers such as virulence factors. Bacteria are currently resisting antimicrobials that are available today, it is only a matter of time before the majority of the population suffers from anti-microbial resistance. Anti-quorum sensing is a medium that can utilize natural compounds for the researchers to be able to conclude that certain organic compounds can have a big impact in terms of having a bactericidal or bacteriostatic effect against these organisms. This study utilized Malunggay ( Moringa Oleifera Lam ) ethanolic leaf extract in selectively blocking acylated Homoserine Lactones (AHL) which regulates the expression of phenotypic characters such as prodigiosin, protease, biofilm formation, and c6-ahl which regulates the hemolysin production, thus inhibiting Serratia marcescens quorum sensing. In this study, the researchers determined the anti-quorum sensing capabilities of the said extract by evaluating the pigment production produced by the bacteria. The researchers utilized 2-fold and 10-fold serial dilutions of the extract in their evaluation. The dilutions were used to determine three parameters namely: Minimum Inhibitory Concentration, Pigment Inhibition, and Minimum Bactericidal Concentration. In this experiment, growth has been observed in all of the dilutions performed in the three parameters. This led to the conclusion that Malunggay (Moringa olifera Lam.) ethanolic leaf extract cannot inhibit the pigment produced by Serratia marcescens.

Thesis - School of Medical Technology

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