In Vitro antibacterial activity of the ethanolic extract of the tuber of dioscorea alata (dioscorides) against pseudomonas aeruginosa / Leslie Vynn S. Qua, Roxanne Loyd N. Quilala, Agnes Christian P. Soriano and Justine Danna Blanch T. Tapulgo.

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Language: english Publication details: Fairview, Quezon City: School of Pharmacy, FEU-NRMF, 2017.Description: 60 pages: illustrations, tables, photos; 28 cm. + CD (soft copy)Content type:

text

Media type:

unmediated

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volume

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LOC classification:

PH 2017 0006

Summary: Abstract: This study was carried out with the objective to determine the antimicrobial activity of the tuber extract of Dioscorea alata (purple yam) by in vitro-disc diffusion assay method against a single cultured bacterial strain of Pseudomonas aeruginosa ATCC 27853. The extraction of Dioscorea alata tuber was performed by using ethanol as the solvent. Paper discs were impregnated with the tuber extract and placed on Mueller - Hinton agar inoculated with Pseudomonas aeruginosa. Mupirocin was used as positive control. The zone of inhibition was calculated by measuring the diameter of the inhibition zone in millimeter using a Vernier caliper. The crude extract tested positive for flavonoids and glycosides. Result for antimicrobial testing found that there was no zone of inhibition against P. aeruginosa.

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Holdings
Item type	Current library	Call number	Status	Notes	Date due	Barcode
Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	PH 2017 0006 (Browse shelf(Opens below))	Available	with CD (soft copy)		T001837

Includes appendices and bibliographical references.

Abstract: This study was carried out with the objective to determine the antimicrobial activity of the tuber extract of Dioscorea alata (purple yam) by in vitro-disc diffusion assay method against a single cultured bacterial strain of Pseudomonas aeruginosa ATCC 27853. The extraction of Dioscorea alata tuber was performed by using ethanol as the solvent. Paper discs were impregnated with the tuber extract and placed on Mueller - Hinton agar inoculated with Pseudomonas aeruginosa. Mupirocin was used as positive control. The zone of inhibition was calculated by measuring the diameter of the inhibition zone in millimeter using a Vernier caliper. The crude extract tested positive for flavonoids and glycosides. Result for antimicrobial testing found that there was no zone of inhibition against P. aeruginosa.

Thesis - School of Pharmacy

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