Oil extract of petroleum nut as potential sealant in HUGH-LEIFSON oxidation-fermentation test, [author]: Arguelles, Jenny Y., Baligod, Elijah B., Bautista, Nicole C., Buenviaje, Rey Michael M., Flores, John Riche L., Lazaro, Kyla Nicole DC., Natal, Michael Jeffrey I., Palomo, Dianne P., Sapal, Bai Hanine R., Villafuerte, Aileen Dominique V., Zason, Patricia Therese B.,

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ABSTRACT:
Pittosporum resiniferum (petroleum nut) has been highlighted in industrial fields as a source of biofuel due to its petroleum-like composition and has been seen as an alternative to mineral oil, a petroleum-based product, utilized as a sealant in hugh-leifson oxidation-fermentation test. The study conducted a series of experiments to provide data on the potential of pertroleum nut (pittosporum resiniferum) oil extract as an alternative sealant to mineral oil. Assessments of color, odor, turbidity, and pH have been employed to assess the quality of the OF test medium sealed with the crude oil extract of petroleum nut, as well as the inoculation of test microorganisms namely eschericha coli, pseudomonas aeruginosa, and bacillus subtilis to determine their compatibility with the medium. The results of assessing the color change of the OF test medium subjected to petroleum nut oil as sealant exhibited mild differences in hues. Being affected by the light passing through the petroleum nut oil located atop of the OF test mediums tubes, the tubes developed mild yellowing atop a dark turbid color while the last tube (labeled S6) exhibit minimal color change. The adapted odor hedonic profile for the assessment of odor implied variable approach to pleasantness of door. The tests for pH and turbidity yielded similar results when compared with oil. The oxidation-fermentation test of the tubes with the petroleum nut oil also yielded similar results with that of the tubes sealed with mineral oil. There is a notable in turbidimetric profile of the inoculated tubes with petroleum nut oil after 24 hours when assessed with the viability of each test microorganisms via spectrophotometer, signifying a metabolic utilization of nutrients in the broth medium thus positive for growth and viability. Utilizing mcnemars test and Wilcoxon-signed rank test, the garnered are statistically similar to those exhibited by the test medium using mineral oil (Z=0.000, p=1.000), with no statistical differences between them. The findings of the study concluded that the oil of petroleum nut could be a potential alternative sealant to mineral oil in Hugh-Leifson oxidation-fermentation test and recommends further approach on subjecting other types of microorganism on the test setup and considering physical and chemical refinement of the plant oil to improve efficacy and eliminate impurities.
Keywords: Microbiology, oil extract, oxidation-fermentation test, petroleum nut, Wilcoxon-signed rank test.

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