Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative /
Jocelyn Q. Gacasan, MD, FPSP
Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative / Patricia Anne Basilio ; Angelo Tadeo Bautista ; Nerrisa Jayne Dalay ; John Joseph Ericson Ermitaño ; Chin Chin Lee ; Zairah Monjardin ; Babylyn Oab ; Diane Eliza Reyes ; Carlo Angelo Rimando ; Niczon See and Mady Ann Grace Serra. - Fairview, Quezon City School of Medical Technology, FEU-NRMF, 2018 - 95 pages: illustration, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Formalin has always been the gold standard fixative in histological preservation. Recently, concerns on its detrimental effect to health has been increasing, such as nasopharyngeal cancer. This urge the researchers to continue searching for a safer alternative. Honey has recently been known to be to fix histological tissues. The only problem with honey is its tendency to attract ants, this is where acetic acid's usage is for. Acetic acid, a known fixative and also an ant repellant, hence a combination of honey and acetic acid is believed to be able to compare with formalin as a fixative. The primary objective of this research was to compare the quality of slides fixed in three different fixatives; namely 10% neutral buffered formalin (NBF), 20% honey with 4% acetic acid. The research used an experimental study which formalin is compared to two other fixatives. With the standard procedure of histological techniques, the pork Tissues were divided into three set-ups and fixed in 10% NBF, 20% honey, or 20% acetic acid. Following fixation is dehydration with increasing grades of ethanol, then clearing with xylene, next is impregnation and embedding with paraffin, and then sectioning with microtome. After sectioning, staining of the tissues with routine H&E follows, and finally mounting is done. Then, the slides are ready for viewing under the microscope. of the 90 slides viewed under the microscope, honey and honey with acetic acid have yielded good results in tissue preservation which is slightly less comparable to that of the gold standard, formalin, which has yielded excellent results both grossly and microscopically; with honey and acetic acid showing better results that that honey alone. Therefore, the researchers encourage the use of honey with acetic acid as a safer alternative to formalin. One that is accessible and health-friendly for the future generation to use.
Thesis - School of Medical Technology
MT 2018 0009
Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative / Patricia Anne Basilio ; Angelo Tadeo Bautista ; Nerrisa Jayne Dalay ; John Joseph Ericson Ermitaño ; Chin Chin Lee ; Zairah Monjardin ; Babylyn Oab ; Diane Eliza Reyes ; Carlo Angelo Rimando ; Niczon See and Mady Ann Grace Serra. - Fairview, Quezon City School of Medical Technology, FEU-NRMF, 2018 - 95 pages: illustration, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Formalin has always been the gold standard fixative in histological preservation. Recently, concerns on its detrimental effect to health has been increasing, such as nasopharyngeal cancer. This urge the researchers to continue searching for a safer alternative. Honey has recently been known to be to fix histological tissues. The only problem with honey is its tendency to attract ants, this is where acetic acid's usage is for. Acetic acid, a known fixative and also an ant repellant, hence a combination of honey and acetic acid is believed to be able to compare with formalin as a fixative. The primary objective of this research was to compare the quality of slides fixed in three different fixatives; namely 10% neutral buffered formalin (NBF), 20% honey with 4% acetic acid. The research used an experimental study which formalin is compared to two other fixatives. With the standard procedure of histological techniques, the pork Tissues were divided into three set-ups and fixed in 10% NBF, 20% honey, or 20% acetic acid. Following fixation is dehydration with increasing grades of ethanol, then clearing with xylene, next is impregnation and embedding with paraffin, and then sectioning with microtome. After sectioning, staining of the tissues with routine H&E follows, and finally mounting is done. Then, the slides are ready for viewing under the microscope. of the 90 slides viewed under the microscope, honey and honey with acetic acid have yielded good results in tissue preservation which is slightly less comparable to that of the gold standard, formalin, which has yielded excellent results both grossly and microscopically; with honey and acetic acid showing better results that that honey alone. Therefore, the researchers encourage the use of honey with acetic acid as a safer alternative to formalin. One that is accessible and health-friendly for the future generation to use.
Thesis - School of Medical Technology
MT 2018 0009
