MARC details
000 -LEADER |
fixed length control field |
03419nam a2200373 4500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
PILC |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20240720153046.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
180927b xxu||||| |||| 00| 0 eng d |
040 ## - CATALOGING SOURCE |
Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
050 ## - LIBRARY OF CONGRESS CALL NUMBER |
Classification number |
MT 2018 0009 |
100 ## - MAIN ENTRY--PERSONAL NAME |
Personal name |
Jocelyn Q. Gacasan, MD, FPSP |
Relator term |
adviser |
111 ## - MAIN ENTRY--MEETING NAME |
Subordinate unit |
RICO 2018 |
245 ## - TITLE STATEMENT |
Title |
Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative / |
Statement of responsibility, etc. |
Patricia Anne Basilio ; Angelo Tadeo Bautista ; Nerrisa Jayne Dalay ; John Joseph Ericson Ermitaño ; Chin Chin Lee ; Zairah Monjardin ; Babylyn Oab ; Diane Eliza Reyes ; Carlo Angelo Rimando ; Niczon See and Mady Ann Grace Serra. |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Place of publication, distribution, etc. |
Fairview, Quezon City |
Name of publisher, distributor, etc. |
School of Medical Technology, FEU-NRMF, |
Date of publication, distribution, etc. |
2018 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
95 pages: |
Other physical details |
illustration, tables, photos; |
Dimensions |
28 cm. |
336 ## - CONTENT TYPE |
Source |
rdacontent |
Content type term |
text |
337 ## - MEDIA TYPE |
Source |
rdamedia |
Media type term |
unmediated |
338 ## - CARRIER TYPE |
Source |
rdacarrier |
Carrier type term |
volume |
504 ## - BIBLIOGRAPHY, ETC. NOTE |
Bibliography, etc |
Includes appendices and bibliographical references. |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Abstract: Formalin has always been the gold standard fixative in histological preservation. Recently, concerns on its detrimental effect to health has been increasing, such as nasopharyngeal cancer. This urge the researchers to continue searching for a safer alternative. Honey has recently been known to be to fix histological tissues. The only problem with honey is its tendency to attract ants, this is where acetic acid's usage is for. Acetic acid, a known fixative and also an ant repellant, hence a combination of honey and acetic acid is believed to be able to compare with formalin as a fixative. The primary objective of this research was to compare the quality of slides fixed in three different fixatives; namely 10% neutral buffered formalin (NBF), 20% honey with 4% acetic acid. The research used an experimental study which formalin is compared to two other fixatives. With the standard procedure of histological techniques, the pork Tissues were divided into three set-ups and fixed in 10% NBF, 20% honey, or 20% acetic acid. Following fixation is dehydration with increasing grades of ethanol, then clearing with xylene, next is impregnation and embedding with paraffin, and then sectioning with microtome. After sectioning, staining of the tissues with routine H&E follows, and finally mounting is done. Then, the slides are ready for viewing under the microscope. of the 90 slides viewed under the microscope, honey and honey with acetic acid have yielded good results in tissue preservation which is slightly less comparable to that of the gold standard, formalin, which has yielded excellent results both grossly and microscopically; with honey and acetic acid showing better results that that honey alone. Therefore, the researchers encourage the use of honey with acetic acid as a safer alternative to formalin. One that is accessible and health-friendly for the future generation to use. |
521 ## - TARGET AUDIENCE NOTE |
Target audience note |
Thesis - School of Medical Technology |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Basilio, Patricia Anne |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Bautista, Angelo Tadeo |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Dalay, Nerrisa Jayne |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Ermitaño, John Joseph Ericson |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Lee, Chin Chin |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Monjardin, Zairah |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Oab, Babylyn |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Reyes, Diane Eliza |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Rimando, Carlo Angelo |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
See, Niczon |
Relator term |
author |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Serra, Mady Ann Grace |
Relator term |
author |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Library of Congress Classification |
Koha item type |
Theses |