Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative / (Record no. 11049)

MARC details
000 -LEADER
fixed length control field 03419nam a2200373 4500
003 - CONTROL NUMBER IDENTIFIER
control field PILC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240720153046.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 180927b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency FEU-NRMF MEDICAL LIBRARY
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number MT 2018 0009
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Jocelyn Q. Gacasan, MD, FPSP
Relator term adviser
111 ## - MAIN ENTRY--MEETING NAME
Subordinate unit RICO 2018
245 ## - TITLE STATEMENT
Title Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative /
Statement of responsibility, etc. Patricia Anne Basilio ; Angelo Tadeo Bautista ; Nerrisa Jayne Dalay ; John Joseph Ericson Ermitaño ; Chin Chin Lee ; Zairah Monjardin ; Babylyn Oab ; Diane Eliza Reyes ; Carlo Angelo Rimando ; Niczon See and Mady Ann Grace Serra.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Fairview, Quezon City
Name of publisher, distributor, etc. School of Medical Technology, FEU-NRMF,
Date of publication, distribution, etc. 2018
300 ## - PHYSICAL DESCRIPTION
Extent 95 pages:
Other physical details illustration, tables, photos;
Dimensions 28 cm.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc Includes appendices and bibliographical references.
520 ## - SUMMARY, ETC.
Summary, etc. Abstract: Formalin has always been the gold standard fixative in histological preservation. Recently, concerns on its detrimental effect to health has been increasing, such as nasopharyngeal cancer. This urge the researchers to continue searching for a safer alternative. Honey has recently been known to be to fix histological tissues. The only problem with honey is its tendency to attract ants, this is where acetic acid's usage is for. Acetic acid, a known fixative and also an ant repellant, hence a combination of honey and acetic acid is believed to be able to compare with formalin as a fixative. The primary objective of this research was to compare the quality of slides fixed in three different fixatives; namely 10% neutral buffered formalin (NBF), 20% honey with 4% acetic acid. The research used an experimental study which formalin is compared to two other fixatives. With the standard procedure of histological techniques, the pork Tissues were divided into three set-ups and fixed in 10% NBF, 20% honey, or 20% acetic acid. Following fixation is dehydration with increasing grades of ethanol, then clearing with xylene, next is impregnation and embedding with paraffin, and then sectioning with microtome. After sectioning, staining of the tissues with routine H&E follows, and finally mounting is done. Then, the slides are ready for viewing under the microscope. of the 90 slides viewed under the microscope, honey and honey with acetic acid have yielded good results in tissue preservation which is slightly less comparable to that of the gold standard, formalin, which has yielded excellent results both grossly and microscopically; with honey and acetic acid showing better results that that honey alone. Therefore, the researchers encourage the use of honey with acetic acid as a safer alternative to formalin. One that is accessible and health-friendly for the future generation to use.
521 ## - TARGET AUDIENCE NOTE
Target audience note Thesis - School of Medical Technology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Basilio, Patricia Anne
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Bautista, Angelo Tadeo
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dalay, Nerrisa Jayne
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Ermitaño, John Joseph Ericson
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Lee, Chin Chin
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Monjardin, Zairah
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Oab, Babylyn
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Reyes, Diane Eliza
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Rimando, Carlo Angelo
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name See, Niczon
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Serra, Mady Ann Grace
Relator term author
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Theses
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation Theses 09/27/2018   MT 2018 0009 c.1 T001976 09/27/2018 09/27/2018 Theses
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation Theses 09/27/2018   MT 2018 0009 c.2 T001977 09/27/2018 09/27/2018 Theses