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Canola oil as a clearing agent in tissue processing / [author]: Aldeza, Patricia Ann S. Arot,Jacquiline G. Bello, Lianne Maxeen S. Estorninos, Ezra T. Gaddi, Sophia Alma R. Galacgac,Jelren Shean I. Manalo, Jewell T. Martinez, Khrystelle Mikaila P. Mediavillo, Shari Chelsea G. Mendoza, Alliah Marie E. Nuestro, Ravel Vincent C. Quisumbing, Mariah Sophia C. Valenzuela, Joy Marie 0. [research adviser]: Reymund M. Mabbagu,

By: Contributor(s): Publication details: Quezon City, Philippines: FEU-Dr. Nicanor Reyes Medical Foundation Institute of Medicine, 2023Description: 106pages / 28cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
LOC classification:
  • MT 2023 0016 c.1
Summary: ABSTRACT: Various studies have stated that employees who are exposed to routinely used clearing agent, Xylene, have a higher risk of having severe Reynaud's phenomenon. Xylene is also categorized as a flammable and toxic substance which requires safety precautions. With this, numerous research studies were conducted to expand the choices of clearing agents with low health risks in order to prevent further exposure to Xylene. This study evaluated Canola oil as a dealcoholization agent in tissue processing. This research adopted an experimental design with a total of 18 samples of pork liver utilized in this study. Nine samples were cleared using Canola Oil while the other nine samples were cleared using the routine clearing agent Xylene as the control. Samples were evaluated based on their Rigidity, Gross Transparency, Gross Shrinkage, and Cellular Architecture as to different time intervals - 30, 60, and 90 minutes. The foundation was the data acquired from the experiment performed in the histopathology laboratory. Canola oil was compared to Xylene (control) and evaluated using the Wilcoxon signed rank test. In summary, the results indicate that at 30, 60, and 90 minutes, there are no significant changes between Canola Oil and Xylene in terms of Rigidity, Gross Transparency, Gross Shrinkage, and Cellular Architecture. Further research with a larger sample size is suggested to enhance the robustness of the conclusive findings regarding the potential utility of Canola Oil in this particular application. Keywords: Canola oil, cellular architecture, clearing agent, gross shrinkage, gross transparency. miscibility, rigidity, xylene
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Theses Far Eastern University - Nicanor Reyes Medical Foundation MT 2023 0016 c.1 (Browse shelf(Opens below)) Available T002749
Theses Far Eastern University - Nicanor Reyes Medical Foundation MT 2023 0016 c.2 (Browse shelf(Opens below)) Available T002750
Theses Far Eastern University - Nicanor Reyes Medical Foundation MT 2023 0016 c.3 (Browse shelf(Opens below)) Available T002751
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MT 2023 0015 c.2 Knowledge and attitudes versus practice: a competency assessment in the use of personal protective equipment among 3rd year students / [author]: Adriano, Angelica Mae I. Alfabeto, Aleah Jane B. Azores, Hannah Czarina Mae Bunag, Dennise EverG. Castillo, Ken Jorelle 0. Contreras, Karl Quenxle S. Dalupang, Charm Angel R. Dela Vega, Kyla Ross J. Ramirez, Christine Faith R. Rapanut, Brigette Threnie R. Ravelo, Ann Gabrielle R. Valguna, Trisha I. [research adviser]: Professor Roberto G. Manaois [research coordinator]: Professor Sherwin Reyes MT 2023 0015 c.3 Knowledge and attitudes versus practice: a competency assessment in the use of personal protective equipment among 3rd year students / [author]: Adriano, Angelica Mae I. Alfabeto, Aleah Jane B. Azores, Hannah Czarina Mae Bunag, Dennise EverG. Castillo, Ken Jorelle 0. Contreras, Karl Quenxle S. Dalupang, Charm Angel R. Dela Vega, Kyla Ross J. Ramirez, Christine Faith R. Rapanut, Brigette Threnie R. Ravelo, Ann Gabrielle R. Valguna, Trisha I. [research adviser]: Professor Roberto G. Manaois [research coordinator]: Professor Sherwin Reyes MT 2023 0016 c.1 Canola oil as a clearing agent in tissue processing / [author]: Aldeza, Patricia Ann S. Arot,Jacquiline G. Bello, Lianne Maxeen S. Estorninos, Ezra T. Gaddi, Sophia Alma R. Galacgac,Jelren Shean I. Manalo, Jewell T. Martinez, Khrystelle Mikaila P. Mediavillo, Shari Chelsea G. Mendoza, Alliah Marie E. Nuestro, Ravel Vincent C. Quisumbing, Mariah Sophia C. Valenzuela, Joy Marie 0. [research adviser]: Reymund M. Mabbagu, MT 2023 0016 c.2 Canola oil as a clearing agent in tissue processing / [author]: Aldeza, Patricia Ann S. Arot,Jacquiline G. Bello, Lianne Maxeen S. Estorninos, Ezra T. Gaddi, Sophia Alma R. Galacgac,Jelren Shean I. Manalo, Jewell T. Martinez, Khrystelle Mikaila P. Mediavillo, Shari Chelsea G. Mendoza, Alliah Marie E. Nuestro, Ravel Vincent C. Quisumbing, Mariah Sophia C. Valenzuela, Joy Marie 0. [research adviser]: Reymund M. Mabbagu, MT 2023 0016 c.3 Canola oil as a clearing agent in tissue processing / [author]: Aldeza, Patricia Ann S. Arot,Jacquiline G. Bello, Lianne Maxeen S. Estorninos, Ezra T. Gaddi, Sophia Alma R. Galacgac,Jelren Shean I. Manalo, Jewell T. Martinez, Khrystelle Mikaila P. Mediavillo, Shari Chelsea G. Mendoza, Alliah Marie E. Nuestro, Ravel Vincent C. Quisumbing, Mariah Sophia C. Valenzuela, Joy Marie 0. [research adviser]: Reymund M. Mabbagu, N ND 0001 Documentary Work Day Care Program NSTP 2 / N ND 0002 c.1 Health Teaching Activities of Staff Nurses in Far Eastern University-Dr. Nicanor Reyes Medical Foundation : An Assessment /

Includes Appendix

ABSTRACT: Various studies have stated that employees who are exposed to routinely used clearing agent, Xylene, have a higher risk of having severe Reynaud's phenomenon. Xylene is also categorized as a flammable and toxic substance which requires safety precautions. With this, numerous research studies were conducted to expand the choices of clearing agents with low health risks in order to prevent further exposure to Xylene. This study evaluated Canola oil as a dealcoholization agent in tissue processing. This research adopted an experimental design with a total of 18 samples of pork liver utilized in this study. Nine samples were cleared using Canola Oil while the other nine samples were cleared using the routine clearing agent Xylene as the control. Samples were evaluated based on their Rigidity, Gross Transparency, Gross Shrinkage, and Cellular Architecture as to different time intervals - 30, 60, and 90 minutes. The foundation was the data acquired from the experiment performed in the histopathology laboratory. Canola oil was compared to Xylene (control) and evaluated using the Wilcoxon signed rank test. In summary, the results indicate that at 30, 60, and 90 minutes, there are no significant changes between Canola Oil and Xylene in terms of Rigidity, Gross Transparency, Gross Shrinkage, and Cellular Architecture. Further research with a larger sample size is suggested to enhance the robustness of the conclusive findings regarding the potential utility of Canola Oil in this particular application. Keywords: Canola oil, cellular architecture, clearing agent, gross shrinkage, gross transparency. miscibility, rigidity, xylene

School of Medical Technology Thesis - School of Medical Technology

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