Citrus maxima (Pomelo) D- limonene volatile oil extract as a deparaffinization agent in histopathology [author]: Carl Allen M. Comaling, Chelsee Yvhon G. Julo, Cris May Bernadette A. Limbaga, Lyca R. Lumaad, Gwyneth Ann P. Masucol, Mark Alexander V. Umipig

Includes Appendix

ABTRACTS:
INTRODUCTION: Xylene is commonly used in laboratories, primarily in histopathology, in order to enhance the transparency of tissue slides. However, it posed significant risks, including flammability and adverse health effects from inhalation or skin exposure. Consequently, the research investigated alternative substances, such as D-limonene
METHOD: Data were collected from observation made by pathologists, employing a methodology that involved manipulating a single variable to examine its effect on another over a nine-month period. The data were organized into a table, and the Mann-Whitney U Test was used to assess the significance and efficacy of the deparaffinization agents. This test was carried out with IBM SPSS, and the p-value was set at 0.05. A pathologist evaluated the parameters using a scoring criterion. The Citrus maxima volatile extract was first obtained using steam distillation and then concentrated through evaporation.
RESULT: The study revealed that the volatile oil, which includes D-limonene, efficiently deparaffinized tissue specimens while Keeping the integrity. The Treated slides were assessed as good to excellent, with a few variations supporting their diagnostic relevance. Based on its efficacy in deparaffinization, Staining quality, and cell morphology preservation, Citrus maxima (Pomelo) D-limonene volatile oil extract appears to be a feasible and perhaps less hazardous clearing agent compared to xylene for tissue processing histopathology.
CONCLUSION: The study emphasized the necessity of investigating less hazardous deparaffinization methods, providing useful insights for medical technology, education, and lowering the health-associated risk with standard reagents such as xylene.
KEYWORDS: Xylene, Mann-Whitney U Test, IBM SPSS, volatile, Steam Distillation evaporation

School of Medical Technology