ANTI-HYPERGLYCEMIC EFFECT OF ORIGANUM VULGARE (OREGANO) CRUDE LEAF EXTRACT WITH A COMPARATIVE STUDY ON ACARBOSE IN VIVO

Oregano vulgare (oregano) crude leaf extract using decoction extraction was compared to Acarbose in vivo, using 10% dextrose hyperglycemia-induced ICR mice. Hyperglycemic state of the test animals was achieved after the results of the mean value of after 10%dextrose oral administration showed significant increase in the serum blood glucose when compared to the levels of glucose in the blood before induction. 1-hour post prandial and 30-min post prandial glucose detrmination test, dubbed as trial I and trial 2 respectively were used to compare anti-hyperglycemic activity relative to time. Using one way-ANOVA test, the results showed a non significant difference of oregano and acarbose at trial 1 however, at trial 2, significant difference were noted. The results would reveal a comparative anti-hyperglycemic effect of crude leaf oregano extract to acarbose only a 1-hour post prandial glucose test using glucose test strip; negative results were noted at 30-min post prandial test. The degree of anti-hyperglycemic activity of oregano is time dependent, as analyzed using paired t-test. This is seen when the mean differences of the samples showed the t-value for trial 1, is above the critical limit of 2,776, which is 7,452 while in trial 2, they t-value is at o.991. However, the degree of anti-hyperglycemic activity of acabose using 1-hr and using 30-min post prandial glucose tests is not limited to the two trials as it showed high efficacy for both. t-values for acarbose in trial 1 and 2 are 5.888 and 3.871, respectively, which are both above critical limit of 2,776. Paired t-test results are using degrees of freedom of 4 at 95% confidence level. Previous researches link these anti-hyperglycemic activities of oregano crude leaf extract to its active components and through the antioxidant properties of soe of its active components, which may intern reduce hyperglycemia and may be of importaance to multiple goals of microvascular and metabolic risks reduction. To end, the oregano crude leaf extract offers a promising potential in the development of a natural remedy in the control of hyperglycemia.

Department of Pharmacology