A RESEARCH ON THE POTENTIATING EFFECTS OF SALICYLATES ON GLIBENCLAMIDE IN LOWERING BLOOD GLUCOSE LEVEL

Pharmacology Thesis

A group of 10 albino rats weighing 200 to 250 gm procured at the Department of Science and Technology was used. They were fasted for 8 hours in preparation for the modified oral glucose tolerance test. After 8 hours of fasting each of them was taken their fasting blood glucose level via glucometer method and this would serve as their baseline level. After which, they were administered 10 % glucose solution ( 4 g/Kg BW ) orally to induce hyperglycemia and the rise of their glucose level was taken again into record by obtaining another blood glucose determination. Blood samples were taken again subsequently with an interval of 30 min. for a span of 120 min. After a 3 day interval, same ten rats was taken their fasting blood sugar after 8 hours fasting and was given 10% glucose solution to induced hyperglycemia. Blood samples were again collected to observe the rise in glucose level. They were given Glibenclamide (2.5mg/Kg BW), an oral hypoglycemic agent and the effect of lowering the blood glucose level is determined by blood collected every 30 min for 120 min. A 3-day interval was given to ensure a total wash out period for the drugs before the next procedure is done. The 10 rats were again induced to hyperglycemia but this were given Glibenclamide with salicylate (30 mg/kg BW) and observed for the results. The results were used to investigate the hypoglycemic effect of Glibenclamide with salicylates as to Glibenclamide when used alone. The results showed that the combination of the two drugs has greater degree of Hypoglycemic effect than Glibenclamide alone.

Department of Pharmacology